The EPFL licenses a novel cationic displacer, its synthesis procedure as well as applications.
Benefits :
¨ Well defined homogeneous structure
¨ Permanently charged
¨ Chemically stable
¨ Homogeneous affinity
Patent status :
Keywords :
Contact researcher / inventor :
Contact Technology Transfer Office :
NOVEL DISPLACER FOR CATION EXCHANGE DISPLACEMENT CHROMATOGRAPHY
The objective of this invention is a special poly(diallyldialkylammonium halogenide)(PDADMAC) type, its synthesis, and its use as displacer in cationic displacement chromatography for the separation of biomolecules. This oligomeric PDADMAC is permanently charged, possesses a homogeneous linear structure as well as a narrow molar mass distribution. Its application allows the separation and concentration of, for example, proteins in a single step. A recovery yield between 80 and 97% was proved in addition to concentration by a factor of 2.5 to 4. Column regeneration can be easily performed.
Potential Commercial applications
Patents pending in US and Europe. PCT application Nr. WO 99/47574 published
Bioseparation, displacement chromatography, cationic displacer, protein displacement.
Prof. Dr. R. Freitag, Dr. habil. C. Wandrey
EPFL-DC-LBTC
CH-1015 Lausanne, Switzerland
e-mail: ruth.freitag@epfl.ch
christine.wandrey@epfl.ch
Tel: +41 21 693 61 62, 21 693 36 72
Fax: +41 21 693 60 30, 21 693 56 90
Benoît Dubuis,PhD, EPFL-SRI
CH-1015 Lausanne, Switzerland
benoit.dubuis@epfl.ch
http://sprwww.epfl.ch/sri
Tel : +41 21 693 35 90
Fax : +41 21 693 70 40
File Nr. 6.0069 (EPFL- SRI)
¨ Separation, purification and concentration of proteins, peptides or antibodies
¨ Cationic displacement chromatography
¨ Separation of multi-component mixtures
