The EPFL licenses a new transfection tools and reagents

Benefits :

¨ The reagents condition the DNA for passing sterile barriers like filters
¨ TGE was demonstrated up to 100-liter scale yielding gram amounts of r-proteins
¨ Fast r-protein expression within days
¨ Low cost
¨ No time critical steps
¨ Straightforward scale-Up

Patent status :
Patent pending

Keywords :

Transient gene expression, Large-scale, fast r-protein expression

Contact researcher / inventor :

Dr. Martin Jordan, Philippe Girard and Prof. Dr. Florian Wurm
EPFL-DC-LBTC
CH-1015 Lausanne, Switzerland
e-mail: philippe.girard@epfl.ch
http://dcwww.epfl.ch/lbtc

Tel: +41 21 693 61 52
Fax: +41 21 693 61 40

Contact Technology Transfer Office :
Benoît Dubuis, EPFL-SRI
CH-1015 Lausanne, Switzerland

benoit.dubuis@epfl.ch
http://sprwww.epfl.ch/sri

Tel : + 41 21 693 35 90
Fax : + 41 21 693 70 40

File Nr. 6.0242 (EPFL SRI)

NOVEL TOOL FOR LARGE-SCALE TRANSIENT GENE EXPRESSION

This new tool allows transferring foreign DNA into cells with an ease not known up to now. As the method does not contain any time critical operation it can be scaled up. The novel method makes it possible to produce with large-scale transient gene expression (TGE) gram amounts of fully transcriptionally modified recombinant proteins in mammalian cell cultures within days. The reagent conditions the DNA for passing sterile barriers guaranteeing sterile transfection and operation. The reagents used are among the most economical available for TGE.
The same technique can be used for screenings of several thousand different DNAs in smaller volume experiments. It can also be used to create stable cell lines.
Overall this novel method can speed up considerably the development of novel proteins for medical applications reducing costs at the same time.