The EPFL licenses a new growth monitoring procedure.
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Can be easily implemented in any fermentation process
¨ No radioactive traces
¨ Proved to be flexible and suitable for different cell lines.
Patent pending US 09/098,284 and PCT/IB99/01092.
Growth monitoring, GFP, biomass, non-invasive.
Prof. Dr. Florian Wurm, Dr. Martin Jordan
EPFL-DC-LBTC
CH-1015 Lausanne, Switzerland
e-mail: martin.jordan@epfl.ch
http://dcwww.epfl.ch/lbtc
Tel: +41 21 693 61 42
Fax: +41 21 693 61 40
Benoît Dubuis, PhD, EPFL-SRI
CH-1015 Lausanne, Switzerland
benoit.dubuis@epfl.ch
http://sprwww.epfl.ch/sri
Tel : +41 21 693 35 90
Fax : +41 21 693 70 40
File Nr. 6.0082 (EPFL- SRI)
ASSAY TO MONITOR GROWTH BY USING GREEN FLUORESCENT CELLS
This invention allows the correlation of the fluorescent signal from stable recombinant cell lines expressing the green fluorescent protein (GFP) at high levels with biomass or cell number, extending the use of fluorescent proteins to applications and assays where cell growth rates are important. Using a standard fluorometer, growth of these cells can be quantified noninvasively in multiwell plates, and because signals are obtained without preparation, the same culture samples can be measured repeatedly. Even with a small relative change in biomass, the specific growth rate can be determined in a few hours. The dynamics of cell populations can now be studied with high sensitivity, low error rate, and minimum sample preparation.
Potential Commercial applications
¨ Growth monitoring at lab and production scale
¨ Media improvement
¨ Process design
