The EPFL licenses a novel digital holography method with applications in microscopy for surface investigations and biology
Benefits :
Patent status :
Keywords :
Contact researcher / inventor :
For applications in biology : Dr.E.Cuche, P.Marquet and Prof.P.Magistretti
Contact Technology Transfer Office :
Absolute phase contrast in microscopy Super-resolution (nm)along the optical axis Opens new fields of application in Biology
US patent pending, PCT application published under Nr. WO 00/20929
Microscopy, holography, phase contrast, surface metrology, cell culture imaging
Dr. C.Depeursinge
EPFL-IOA-DMT
CH-1015 Lausanne, Switzerland
e-mail: christian.depeursinge@epfl.ch
Tel: +41 21 693 61 77
Fax: +41 21 693 37 01
UNIL-IP
André Catana, PhD, EPFL-SRI
CH-1015 Lausanne, Switzerland
andre.catana@epfl.ch
http://sprwww.epfl.ch/sri
Tel : + 41 21 693 55 81
Fax : + 41 21 693 70 40
File Nr. 6.0147 (EPFL- SRI)
DIGITAL HOLOGRAPHY APPLIED TO MICROSCOPY
A method and apparatus for the numerical reconstruction of digital holograms which allows simultaneous amplitude and quantitative phase contrast imaging. The reconstruction method computes the propagation of the complex optical wavefront emitted by the object and may serve to determine the refractive index and/or shape of the object with an accuracy in the nanometer range along the optical axis. The method requires the adjustment of several reconstruction parameters which is performed automatically. The method also includes a digital method for the correction of the optical aberrations. It finds diverse and important applications in microscopy: surface studies, metrology and biological applications for the determination of cell volumes and refractive index
Potential Commercial applications
¨ Surface investigations, wafer inspection
¨ Topology, profilometry, rugosity measurements
¨ Absolute phase contrast microscopy in biology
